Journal: Virchows Archiv : an international journal of pathology

Article Title: Toll-like receptors 1, 2, 4, 5, and 6 in gastric cancer.

doi: 10.1007/s00428-023-03635-1

Figure Lengend Snippet: Fig. 1 Representative images of cytoplasmic TLR2 and TLR6 expression immunostaining in gastric adenocarcinoma. Low cytoplasmic TLR2 expression (A), high cytoplasmic TLR2 expression (B), low cytoplasmic TLR6 expression (C), and high cytoplasmic TLR6 expression (D)

Article Snippet: Samples were rinsed in distilled water and phosphate-buffered saline with Tween (PBS-T) and endogenous peroxidase was then neutralized in peroxidase blocking solution (Dako S2023) for 5 min. After a wash in PBS-T, sections were incubated with antibodies (Dako S2022); TLR1 (diluted 1:300, ROCKLAND 800-656-7625), TLR2 (diluted 1:500, ROCKLAND 600-401-956), TLR4 (diluted 1:1000, abonova 214-291), TLR5 (1:75, NOVUS NBP-2-24787), and TLR6 (diluted 1:750, abonova PAB 3555).

Techniques: Expressing, Immunostaining

Journal: bioRxiv

Article Title: Multivalent Targeting of Blood-Brain Barrier LRP1 for Neurovascular Recovery Therapy for Alzheimer’s Disease

doi: 10.1101/2024.05.06.592767

Figure Lengend Snippet: Quantitative representation of Aβ-positive areas ( a ) in brain coronal sections, identified through immune histochemistry (IHC), in both AD model and wild-type mice. Quantitative measurement of Aβ concentrations via ELISA in both AD and wild type mice of the whole-brain ( b ), its vasculature ( c ) and parenchyma ( d ). ELISA quantification of both brain vasculature and parenchyma ( e ) in AD and wild type mice of key proteins involved in trafficking (LRP1, PACSIN2, RAB5), metabolism (GLUT1), tight junction proteins (ZO-1, CLDN11), and other stress-related proteins (MBP, MAP-2, SRB1). For all analyzes (n = 3)

Article Snippet: Protein concentrations for various proteins, including LRP1 (NOVUS, NBP3-00449), PACSIN2 (Anruike, YX-160103M), RAB5 (abbexa, abx154598), GLUT1 (Anruike, YX-071242M), Claudin11 (Anruike, YX-031229M), ZO1 (Elabscience, E-EL-M1161), MBP (Elabscience, E-EL-M0805), MAP-2 (Anruike, YX-120118M), SRB1 (Anruike, ARK-756321A), and Aβ (invitrogen, KMB3441) were determined using ELISA kits following the manufacturer protocols.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Multivalent Targeting of Blood-Brain Barrier LRP1 for Neurovascular Recovery Therapy for Alzheimer’s Disease

doi: 10.1101/2024.05.06.592767

Figure Lengend Snippet: Pearson correlation coefficients for the colocalization of LRP1 and BBB endothelial cells (CD31) pre- and post A 39 -POs administration ( a ). Analysis performed on images derived from three independent experiments, with 6-7 vessels studied per trial. Quantification of Aβ content in cerebrovascular endothelial cells and brain parenchyma ( b ) (n = 3, statistical analyzis performed using unpaired t-tests, **** p<0.0001). ELISA measurements ( c ) of vascular and parenchymal proteins in 12-month-old WT, Sham APP/PS1, and APP/PS1 post A 39 -POs treatment mice, encompassing LRP1, PACSIN2, RAB5, GLUT1, Claudin 11, ZO-1, MBP, MAP-2, and SRB1. STED microscopy ( d ) of LRP1 (white) on the vessel wall (green), indicative of active transcytosis. Post-treatment, Aβ deposits around the BBB (red) are cleared with notable Aβ signal presence within the vascular lumen. For (b) and (c), statistical significance determined using one-way ANOVA, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, n ≥ 3.

Article Snippet: Protein concentrations for various proteins, including LRP1 (NOVUS, NBP3-00449), PACSIN2 (Anruike, YX-160103M), RAB5 (abbexa, abx154598), GLUT1 (Anruike, YX-071242M), Claudin11 (Anruike, YX-031229M), ZO1 (Elabscience, E-EL-M1161), MBP (Elabscience, E-EL-M0805), MAP-2 (Anruike, YX-120118M), SRB1 (Anruike, ARK-756321A), and Aβ (invitrogen, KMB3441) were determined using ELISA kits following the manufacturer protocols.

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Microscopy